Transposition of IS50L activates downstream genes

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Transposition of IS50L activates downstream genes.

A transposition system constructed to detect the transposition of Tn5 to a site upstream of the lacZ gene has revealed that transposition of IS50L can activate downstream genes. Expression is apparently mediated by the NPTII promoter. Transposase produced either by IS50R or by the suppressed IS50L catalyzed transposition of IS50L.

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Skeletal muscle denervation activates acetylcholine receptor genes

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Skeletal Muscle Denervation Activates Acetylcholine Receptor Genes

Transcriptional activity of acetylcholine receptor subunit genes was investigated in innervated and denervated chick skeletal muscle. The sciatic nerve of 3-d-old White Leghorn chicks was sectioned unilaterally; after various intervals, nuclei were isolated from operated and sham-operated animals, and run-on assays performed. Nuclei were incubated with 32p-UTP, and total RNA was extracted and h...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1988

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.170.4.1965-1968.1988